• Western blot analysis of extracts of various cell lines, using B9D1 antibody (STJ110280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using B9D1 Rabbit polyclonal antibody (STJ110280) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human stomach using B9D1 antibody (STJ110280) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of L929 cells using B9D1 Rabbit polyclonal antibody (STJ110280) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat kidney using B9D1 antibody (STJ110280) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using B9D1 antibody (STJ110280) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse lung using B9D1 antibody (STJ110280) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using B9D1 antibody (STJ110280) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using B9D1 rabbit polyclonal antibody (STJ110280) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using B9D1 antibody (STJ110280) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using B9D1 rabbit polyclonal antibody (STJ110280) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using B9D1 antibody (STJ110280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-B9D1 antibody (1-130) (STJ110280)

SKU:
STJ110280

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-B9D1 (1-130) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: B9D1
Gene ID: 27077
Uniprot ID: B9D1_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human B9D1 (NP_056496.1).
Immunogen Sequence: MATASPSVFLLMVNGQVESA QFPEYDDLYCKYCFVYGQDW APTAGLEEGISQITSKSQDV RQALVWNFPIDVTFKSTNPY GWPQIVLSVYGPDVFGNDVV RGYGAVHVPFSPGRHKRTIP MFVPESTSKL
Function Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling.
Protein Name B9 Domain-Containing Protein 1
Mks1-Related Protein 1
Database Links Reactome: R-HSA-5620912
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Basal Body
Cilium Axoneme
Localizes At The Transition Zone
A Region Between The Basal Body And The Ciliary Axoneme
Alternative Antibody Names Anti-B9 Domain-Containing Protein 1 antibody
Anti-Mks1-Related Protein 1 antibody
Anti-B9D1 antibody
Anti-MKSR1 antibody

Information sourced from Uniprot.org

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