• STJ71250 (1ug/ml) staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C) , Human Adrenal Gland iso 2 (D) and (0. 1µg/ml) Human pancreas iso 1 (E) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71250 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane/cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71250 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-B7-H4 antibody (Internal) (STJ71250)

SKU:
STJ71250-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-B7-H4 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: VTCN1
Gene ID: 79679
Uniprot ID: VTCN1_HUMAN
Immunogen Region: Internal
Accession Number: NP_078902.2; NP_001240778.1
Immunogen Sequence: SKGKGNANLEYK
Post Translational Modifications N-glycosylated.
Function Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
Protein Name V-Set Domain-Containing T-Cell Activation Inhibitor 1
B7 Homolog 4
B7-H4
B7h.5
Immune Costimulatory Protein B7-H4
Protein B7s1
T-Cell Costimulatory Molecule B7x
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Expressed At The Cell Surface
A Soluble Form Has Also Been Detected
Alternative Antibody Names Anti-V-Set Domain-Containing T-Cell Activation Inhibitor 1 antibody
Anti-B7 Homolog 4 antibody
Anti-B7-H4 antibody
Anti-B7h.5 antibody
Anti-Immune Costimulatory Protein B7-H4 antibody
Anti-Protein B7s1 antibody
Anti-T-Cell Costimulatory Molecule B7x antibody
Anti-VTCN1 antibody
Anti-B7H4 antibody
Anti-UNQ659 antibody
Anti-PRO1291 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance