Host: |
Goat |
Applications: |
Pep-ELISA/IHC |
Reactivity: |
Human/Mouse/Rat/Dog/Cow |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Goat polyclonal antibody anti-AXIN1 (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
Clonality: |
Polyclonal |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: |
0.5 mg/mL |
Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave bands at approx 150kDa and 22-24kDa in Human Brain (Hippocampus) lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an ex |
Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: |
AXIN1 |
Gene ID: |
8312 |
Uniprot ID: |
AXIN1_HUMAN |
Immunogen Region: |
C-Term |
Accession Number: |
NP_003493.1; NP_851393.1 |
Specificity: |
This antibody is expected to recognise both reported isoforms. |
Immunogen Sequence: |
FEEKIIGKVEKVD |
Post Translational Modifications | Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1. ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. Ubiquitination by SIAH1 and SIAH2 induces its proteasomal degradation as part of the activation of the Wnt signaling pathway. |
Function | Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects.down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also a component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. |
Protein Name | Axin-1Axis Inhibition Protein 1Haxin |
Database Links | Reactome: R-HSA-195253Reactome: R-HSA-196299Reactome: R-HSA-201681Reactome: R-HSA-4641257Reactome: R-HSA-4641262Reactome: R-HSA-5339716Reactome: R-HSA-5358747Reactome: R-HSA-5358749Reactome: R-HSA-5358751Reactome: R-HSA-5358752Reactome: R-HSA-5467337Reactome: R-HSA-5467340Reactome: R-HSA-5467348Reactome: R-HSA-5689880Reactome: R-HSA-8931987Reactome: R-HSA-8939256Reactome: R-HSA-9018519 |
Cellular Localisation | CytoplasmNucleusMembraneCell MembraneMacf1 Is Required For Its Translocation To Cell MembraneOn Uv IrradiationTranslocates To The Nucleus And Colocalizes With Daax |
Alternative Antibody Names | Anti-Axin-1 antibodyAnti-Axis Inhibition Protein 1 antibodyAnti-Haxin antibodyAnti-AXIN1 antibodyAnti-AXIN antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance