Anti-AUTS2 antibody (550-770) (STJ11103329)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal AUTS2 (550-770) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AUTS2 |
| Gene ID | 26053 |
| Uniprot ID | AUTS2_HUMAN |
| Immunogen Region | 550-770 |
| Immunogen Sequence | IPPTAIMPTPAPPMFDKYPT KVDPFYRHSLFHSYPPAVSG IPPMIPPTGPFGSLQGAFQP KLTDPFRPMLRKPGKWCAMH VHIAWQIYHHQQKVKKQMQS DPHKLDFGLKPEFLSRPPGP SLFGAIHHPHDLARPSTLFS AAGAAHPTGTPFGPPPHHSN FLNPAAHLEPFNRPSTFTGL AAVGGNAFGGLGNPSVTPNS MFGHKDGPSVQNFSNPHEPW N |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 550-770 of human AUTS2 (NP_001120703). |
Additional Info
| Tissue Specificity | Strongly expressed in brain, skeletal muscle and kidney. Also expressed in placenta, lung and leukocytes. |
| Function | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The PRC1-like complex that contains PCGF5, RNF2, CSNK2B, RYBP and AUTS2 has decreased histone H2A ubiquitination activity, due to the phosphorylation of RNF2 by CSNK2B. As a consequence, the complex mediates transcriptional activation. In the cytoplasm, plays a role in axon and dendrite elongation and in neuronal migration during embryonic brain development. Promotes reorganization of the actin cytoskeleton, lamellipodia formation and neurite elongation via its interaction with RAC guanine nucleotide exchange factors, which then leads to the activation of RAC1. |
| Protein Name | Autism Susceptibility Gene 2 Protein |
| Database Links | Reactome: R-HSA-8939243 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonCell ProjectionGrowth ConeDetected Both In Cytoplasm And NucleusColocalizes With Rac1 At Actin-Rich Growth ConesDetected On The Promoter Region Of Actively Transcribed Genes |
| Alternative Antibody Names | Anti-Autism Susceptibility Gene 2 Protein antibodyAnti-AUTS2 antibodyAnti-KIAA0442 antibody |
Information sourced from Uniprot.org