• Immunofluorescence analysis of U2OS cells using AUTS2 Rabbit polyclonal antibody (STJ11103329) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using AUTS2 Rabbit polyclonal antibody (STJ11103329) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using AUTS2 Rabbit polyclonal antibody (STJ11103329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-AUTS2 antibody (550-770) (STJ11103329)

SKU:
STJ11103329

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AUTS2 (550-770) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AUTS2
Gene ID: 26053
Uniprot ID: AUTS2_HUMAN
Immunogen Region: 550-770
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 550-770 of human AUTS2 (NP_001120703).
Immunogen Sequence: IPPTAIMPTPAPPMFDKYPT KVDPFYRHSLFHSYPPAVSG IPPMIPPTGPFGSLQGAFQP KLTDPFRPMLRKPGKWCAMH VHIAWQIYHHQQKVKKQMQS DPHKLDFGLKPEFLSRPPGP SLFGAIHHPHDLARPSTLFS AAGAAHPTGTPFGPPPHHSN FLNPAAHLEPFNRPSTFTGL AAVGGNAFGGLGNPSVTPNS MFGHKDGPSVQNFSNPHEPW N
Tissue Specificity Strongly expressed in brain, skeletal muscle and kidney. Also expressed in placenta, lung and leukocytes.
Function Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The PRC1-like complex that contains PCGF5, RNF2, CSNK2B, RYBP and AUTS2 has decreased histone H2A ubiquitination activity, due to the phosphorylation of RNF2 by CSNK2B. As a consequence, the complex mediates transcriptional activation. In the cytoplasm, plays a role in axon and dendrite elongation and in neuronal migration during embryonic brain development. Promotes reorganization of the actin cytoskeleton, lamellipodia formation and neurite elongation via its interaction with RAC guanine nucleotide exchange factors, which then leads to the activation of RAC1.
Protein Name Autism Susceptibility Gene 2 Protein
Database Links Reactome: R-HSA-8939243
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Cell Projection
Growth Cone
Detected Both In Cytoplasm And Nucleus
Colocalizes With Rac1 At Actin-Rich Growth Cones
Detected On The Promoter Region Of Actively Transcribed Genes
Alternative Antibody Names Anti-Autism Susceptibility Gene 2 Protein antibody
Anti-AUTS2 antibody
Anti-KIAA0442 antibody

Information sourced from Uniprot.org

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