| Host: |
Rabbit |
| Applications: |
WB/IHC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Lipid Droplet-Regulating Vldl Assembly Factor Aup1 is suitable for use in Western Blot, Immunohistochemistry and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Purification: |
Affinity purification |
| Concentration: |
0.4 mg/mL |
| Dilution Range: |
WB 1:500-1:2000, IHC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Gene Symbol: |
AUP1 |
| Gene ID: |
550 |
| Uniprot ID: |
AUP1_HUMAN |
| Immunogen: |
Recombinant protein of human AUP1 |
| Tissue Specificity | Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain. |
| Post Translational Modifications | Monoubiquitinated and diubiquitinated. (Microbial infection) Not ubiquitinated following Dengue virus infection. |
| Function | Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Plays a role in lipid droplet formation. Induces lipid droplet clustering. Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation. Also required for the degradation of INSIG1, SREBF1 and SREBF2. Plays a role in regulating assembly and secretion of very low density lipoprotein particles and stability of apolipoprotein APOB. (Microbial infection) Following Dengue virus infection, required for induction of lipophagy which facilitates production of virus progeny particles. |
| Protein Name | Lipid Droplet-Regulating Vldl Assembly Factor Aup1Ancient Ubiquitous Protein 1 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinLipid DropletCytoplasmic VesicleAutophagosome(Microbial Infection) Upon Dengue Virus InfectionRelocates From Lipid Droplets To Autophagosomes |
| Alternative Antibody Names | Anti-Lipid Droplet-Regulating Vldl Assembly Factor Aup1 antibodyAnti-Ancient Ubiquitous Protein 1 antibodyAnti-AUP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
