| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-ATP8A1 (550-600 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | ATP8A1 |
| Gene ID: | 10396 |
| Uniprot ID: | AT8A1_HUMAN |
| Immunogen Region: | 550-600 aa |
| Specificity: | Specific for ATP8A1. |
| Immunogen: | A synthetic peptide from aa region 550-600 of human ATP8A1 conjugated to an immunogenic carrier protein was used as the antigen. |
| Post Translational Modifications | Cleaved by calpain in a caspase- and calcium influx-dependent manner during platelet apoptosis leading to a 100 kDa polypeptide. |
| Function | Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS). The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the cell membrane. Acts as aminophospholipid translocase at the cell membrane in neuronal cells. |
| Protein Name | Phospholipid-Transporting Atpase Ia Atpase Class I Type 8a Member 1 Chromaffin Granule Atpase Ii P4-Atpase Flippase Complex Alpha Subunit Atp8a1 |
| Database Links | Reactome: R-HSA-6798695 Reactome: R-HSA-936837 |
| Cellular Localisation | Cytoplasmic Vesicle Secretory Vesicle Chromaffin Granule Membrane Multi-Pass Membrane Protein Cytoplasmic Granule Cell Membrane Endoplasmic Reticulum Golgi Apparatus Exit From The Endoplasmic Reticulum Requires The Presence Of Tmem30a But Not Tmem30b In The Presence Of Tmem30a Predominantly Located In Cytoplasmic Punctate Structures And Localizes To The Cell Membrane Localizes To Plasma Membranes Of Red Blood Cells |
| Alternative Antibody Names | Anti-Phospholipid-Transporting Atpase Ia antibody Anti-Atpase Class I Type 8a Member 1 antibody Anti-Chromaffin Granule Atpase Ii antibody Anti-P4-Atpase Flippase Complex Alpha Subunit Atp8a1 antibody Anti-ATP8A1 antibody Anti-ATPIA antibody |
Information sourced from Uniprot.org