Anti-ATP8A1 antibody (159-209 aa) (STJ194597)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human AT8A1 at the amino acid range 159-209
General Information
| Short Description | Rabbit polyclonal anti-Phospholipid-transporting ATPase IA (159-209 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP8A1 |
| Gene ID | 10396 |
| Uniprot ID | AT8A1_HUMAN |
| Immunogen | Synthesized peptide derived from the human AT8A1 at the amino acid range 159-209 |
| Immunogen Region | 159-209 aa |
| Specificity | This antibody detects endogenous levels of AT8A1 at Human/Mouse |
Additional Info
| Post Translational Modifications | Cleaved by calpain in a caspase- and calcium influx-dependent manner during platelet apoptosis leading to a 100 kDa polypeptide. |
| Function | Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation also seems to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS). The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the cell membrane. Acts as aminophospholipid translocase at the cell membrane in neuronal cells. |
| Protein Name | Phospholipid-Transporting Atpase IaAtpase Class I Type 8a Member 1Chromaffin Granule Atpase IiP4-Atpase Flippase Complex Alpha Subunit Atp8a1 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-936837 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleChromaffin Granule MembraneMulti-Pass Membrane ProteinCytoplasmic GranuleCell MembraneEndoplasmic ReticulumGolgi ApparatusExit From The Endoplasmic Reticulum Requires The Presence Of Tmem30aBut Not Tmem30bIn The Presence Of Tmem30aPredominantly Located In Cytoplasmic Punctate Structures And Localizes To The Cell MembraneLocalizes To Plasma Membranes Of Red Blood Cells |
| Alternative Antibody Names | Anti-Phospholipid-Transporting Atpase Ia antibodyAnti-Atpase Class I Type 8a Member 1 antibodyAnti-Chromaffin Granule Atpase Ii antibodyAnti-P4-Atpase Flippase Complex Alpha Subunit Atp8a1 antibodyAnti-ATP8A1 antibodyAnti-ATPIA antibody |
Information sourced from Uniprot.org