Anti-ATP6V1E1 antibody (77-226) (STJ22732)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP6V1E1 (77-226) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP6V1E1 |
| Gene ID | 529 |
| Uniprot ID | VATE1_HUMAN |
| Immunogen Region | 77-226 |
| Immunogen Sequence | NQARLKVLRARDDLITDLLN EAKQRLSKVVKDTTRYQVLL DGLVLQGLYQLLEPRMIVRC RKQDFPLVKAAVQKAIPMYK IATKNDVDVQIDQESYLPED IAGGVEIYNGDRKIKVSNTL ESRLDLIAQQMMPEVRGALF GANANRKFLD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 77-226 of human ATP6V1E1 (NP_001687.1). |
Additional Info
| Tissue Specificity | Kidney.localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level). Ubiquitous. High expression in the skin. |
| Function | Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. |
| Protein Name | V-Type Proton Atpase Subunit E 1V-Atpase Subunit E 1V-Atpase 31 Kda SubunitP31Vacuolar Proton Pump Subunit E 1 |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-77387Reactome: R-HSA-917977Reactome: R-HSA-9639288Reactome: R-HSA-983712Reactome: R-HSA-9857377 |
| Cellular Localisation | Apical Cell MembranePeripheral Membrane ProteinCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneClathrin-Coated Vesicle Membrane |
| Alternative Antibody Names | Anti-V-Type Proton Atpase Subunit E 1 antibodyAnti-V-Atpase Subunit E 1 antibodyAnti-V-Atpase 31 Kda Subunit antibodyAnti-P31 antibodyAnti-Vacuolar Proton Pump Subunit E 1 antibodyAnti-ATP6V1E1 antibodyAnti-ATP6E antibodyAnti-ATP6E2 antibody |
Information sourced from Uniprot.org