Anti-ATP6V1B1 antibody (1-280) (STJ28956)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP6V1B1 (1-280) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP6V1B1 |
| Gene ID | 525 |
| Uniprot ID | VATB1_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MAMEIDSRPGGLPGSSCNLG AAREHMQAVTRNYITHPRVT YRTVCSVNGPLVVLDRVKFA QYAEIVHFTLPDGTQRSGQV LEVAGTKAIVQVFEGTSGID ARKTTCEFTGDILRTPVSED MLGRVFNGSGKPIDKGPVVM AEDFLDINGQPINPHSRIYP EEMIQTGISPIDVMNSIARG QKIPIFSAAGLPHNEIAAQI CRQAGLVKKSKAVLDYHDDN FAIVFAAMGVNMETARFFK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP6V1B1 (NP_001683.2). |
Additional Info
| Tissue Specificity | Kidney.localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level). Expressed in the cochlea and endolymphatic sac. |
| Function | Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium. |
| Protein Name | V-Type Proton Atpase Subunit B - Kidney IsoformV-Atpase Subunit B 1Endomembrane Proton Pump 58 Kda SubunitVacuolar Proton Pump Subunit B 1 |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-77387Reactome: R-HSA-917977Reactome: R-HSA-9639288Reactome: R-HSA-983712 |
| Cellular Localisation | Apical Cell MembraneBasolateral Cell Membrane |
| Alternative Antibody Names | Anti-V-Type Proton Atpase Subunit B - Kidney Isoform antibodyAnti-V-Atpase Subunit B 1 antibodyAnti-Endomembrane Proton Pump 58 Kda Subunit antibodyAnti-Vacuolar Proton Pump Subunit B 1 antibodyAnti-ATP6V1B1 antibodyAnti-ATP6B1 antibodyAnti-VATB antibodyAnti-VPP3 antibody |
Information sourced from Uniprot.org