Anti-ATP6AP1 antibody [SRM14535] (STJ11109265)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal ATP6AP1 antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14535 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:5000IHC-P, 1:100-1:500ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP6AP1 |
| Gene ID | 537 |
| Uniprot ID | VAS1_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | widely expressed, with highest levels in brain and lowest in liver and duodenum. |
| Post Translational Modifications | N-glycosylated. |
| Function | Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+)-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex (Probable). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. In islets of Langerhans cells, may regulate the acidification of dense-core secretory granules. |
| Protein Name | V-Type Proton Atpase Subunit S1V-Atpase Subunit S1Protein Xap-3V-Atpase Ac45 SubunitV-Atpase S1 Accessory ProteinVacuolar Proton Pump Subunit S1 |
| Database Links | Reactome: R-HSA-77387Reactome: R-HSA-8980692Reactome: R-HSA-917977Reactome: R-HSA-983712 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum-Golgi Intermediate Compartment MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneClathrin-Coated Vesicle MembraneNot Detected In Trans-Golgi Network |
| Alternative Antibody Names | Anti-V-Type Proton Atpase Subunit S1 antibodyAnti-V-Atpase Subunit S1 antibodyAnti-Protein Xap-3 antibodyAnti-V-Atpase Ac45 Subunit antibodyAnti-V-Atpase S1 Accessory Protein antibodyAnti-Vacuolar Proton Pump Subunit S1 antibodyAnti-ATP6AP1 antibodyAnti-ATP6IP1 antibodyAnti-ATP6S1 antibodyAnti-VATPS1 antibodyAnti-XAP3 antibody |
Information sourced from Uniprot.org