Anti-ATP5O antibody (24-213) (STJ110402)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP5O (24-213) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP5PO |
| Gene ID | 539 |
| Uniprot ID | ATPO_HUMAN |
| Immunogen Region | 24-213 |
| Immunogen Sequence | FAKLVRPPVQVYGIEGRYAT ALYSAASKQNKLEQVEKELL RVAQILKEPKVAASVLNPYV KRSIKVKSLNDITAKERFSP LTTNLINLLAENGRLSNTQG VVSAFSTMMSVHRGEVPCTV TSASPLEEATLSELKTVLKS FLSQGQVLKLEAKTDPSILG GMIVRIGEKYVDMSVKTKIQ KLGRAMREIV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 24-213 of human ATP5O (NP_001688.1). |
Additional Info
| Post Translational Modifications | Acetylation at Lys-162 decreases ATP production. Deacetylated by SIRT3. In response to mitochondrial stress, the precursor protein is ubiquitinated by the SIFI complex in the cytoplasm before mitochondrial import, leading to its degradation. Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1. |
| Function | Subunit OSCP, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. |
| Protein Name | Atp Synthase Peripheral Stalk Subunit Oscp - MitochondrialAtp Synthase Subunit OOligomycin Sensitivity Conferral ProteinOscp |
| Database Links | Reactome: R-HSA-163210Reactome: R-HSA-8949613Reactome: R-HSA-9837999 |
| Cellular Localisation | MitochondrionMitochondrion Inner Membrane |
| Alternative Antibody Names | Anti-Atp Synthase Peripheral Stalk Subunit Oscp - Mitochondrial antibodyAnti-Atp Synthase Subunit O antibodyAnti-Oligomycin Sensitivity Conferral Protein antibodyAnti-Oscp antibodyAnti-ATP5PO antibodyAnti-ATP5O antibodyAnti-ATPO antibody |
Information sourced from Uniprot.org