Anti-ATP5C1 antibody (C-Term) (STJ73419)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ATP5C1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 33kDa band observed in Human Heart and in Rat Heart lysates (calculated MW of 32.9kDa according to NP_005165.1). Recommended concentration: 0.01-0.03ยตg/ml.IHC: Paraffin em |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP5F1C |
| Gene ID | 509 |
| Uniprot ID | ATPG_HUMAN |
| Accession Number | NP_005165.1; NP_001001973.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | TLTFNRTRQAVITKE |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_005165.1; NP_001001973.1). |
Additional Info
| Function | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
| Protein Name | Atp Synthase Subunit Gamma - MitochondrialAtp Synthase F1 Subunit GammaF-Atpase Gamma Subunit |
| Database Links | Reactome: R-HSA-163210Reactome: R-HSA-8949613Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Atp Synthase Subunit Gamma - Mitochondrial antibodyAnti-Atp Synthase F1 Subunit Gamma antibodyAnti-F-Atpase Gamma Subunit antibodyAnti-ATP5F1C antibodyAnti-ATP5C antibodyAnti-ATP5C1 antibodyAnti-ATP5CL1 antibody |
Information sourced from Uniprot.org