Anti-ATP4B antibody (62-291) (STJ112145)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP4B (62-291) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP4B |
| Gene ID | 496 |
| Uniprot ID | ATP4B_HUMAN |
| Immunogen Region | 62-291 |
| Immunogen Sequence | LMQTVDPYTPDYQDQLRSPG VTLRPDVYGEKGLEIVYNVS DNRTWADLTQTLHAFLAGYS PAAQEDSINCTSEQYFFQES FRAPNHTKFSCKFTADMLQN CSGLADPNFGFEEGKPCFII KMNRIVKFLPSNGSAPRVDC AFLDQPRELGQPLQVKYYPP NGTFSLHYFPYYGKKAQPHY SNPLVAAKLLNIPRNAEVAI VCKVMAEHVTFNNPHDPYEG KVEFKLKIEK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 62-291 of human ATP4B (NP_000696.1). |
Additional Info
| Post Translational Modifications | N-glycosylation is necessary for assembly and functional expression of the pump at the plasma membrane. |
| Function | The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle. |
| Protein Name | Potassium-Transporting Atpase Subunit BetaGastric H(+/K(+ Atpase Subunit BetaProton Pump Beta Chain |
| Database Links | Reactome: R-HSA-936837 |
| Cellular Localisation | Apical Cell MembraneSingle-Pass Type Ii Membrane ProteinCell MembraneLocalized In The Apical Canalicular Membrane Of Parietal Cells |
| Alternative Antibody Names | Anti-Potassium-Transporting Atpase Subunit Beta antibodyAnti-Gastric H(+/K(+ Atpase Subunit Beta antibodyAnti-Proton Pump Beta Chain antibodyAnti-ATP4B antibody |
Information sourced from Uniprot.org