| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Potassium-transporting ATPase alpha chain 1/K+ ATPase subunit alpha (800-880 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ATP4A |
| Gene ID: | 495 |
| Uniprot ID: | ATP4A_HUMAN |
| Immunogen Region: | 800-880 aa |
| Specificity: | ATP4A Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 800-880 |
| Function | The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state. |
| Protein Name | Potassium-Transporting Atpase Alpha Chain 1 Gastric H(+/K(+ Atpase Subunit Alpha Proton Pump |
| Database Links | Reactome: R-HSA-936837 |
| Cellular Localisation | Apical Cell Membrane Multi-Pass Membrane Protein Localized In The Apical Canalicular Membrane Of Parietal Cells |
| Alternative Antibody Names | Anti-Potassium-Transporting Atpase Alpha Chain 1 antibody Anti-Gastric H(+/K(+ Atpase Subunit Alpha antibody Anti-Proton Pump antibody Anti-ATP4A antibody |
Information sourced from Uniprot.org