| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-ATP12A (N-Term) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | ATP12A |
| Gene ID: | 479 |
| Uniprot ID: | AT12A_HUMAN |
| Immunogen Region: | N-Term |
| Specificity: | Specific for ATP12A. |
| Immunogen: | A synthetic peptide from n-terminal region of human ATP12A conjugated to blue carrier protein was used as the antigen. |
| Function | The catalytic subunit of a H(+)/K(+) ATPase and/or Na(+)/K(+) ATPase pump which transports K(+) ions in exchange for Na(+) and/or H(+) ions across the apical membrane of epithelial cells. Uses ATP as an energy source to pump K(+) ions into the cell while transporting Na(+) and/or H(+) ions to the extracellular compartment. Involved in the maintenance of electrolyte homeostasis through K(+) ion absorption in kidney and colon. In the airway epithelium, may play a primary role in mucus acidification regulating its viscosity and clearance. |
| Protein Name | Potassium-Transporting Atpase Alpha Chain 2Hk Alpha 2Non-Gastric H(+/K(+ Atpase Subunit AlphaNon-Gastric Na(+/K(+ Atpase Subunit AlphaProton PumpSodium Pump |
| Database Links | Reactome: R-HSA-936837 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Potassium-Transporting Atpase Alpha Chain 2 antibodyAnti-Hk Alpha 2 antibodyAnti-Non-Gastric H(+/K(+ Atpase Subunit Alpha antibodyAnti-Non-Gastric Na(+/K(+ Atpase Subunit Alpha antibodyAnti-Proton Pump antibodyAnti-Sodium Pump antibodyAnti-ATP12A antibodyAnti-ATP1AL1 antibody |
Information sourced from Uniprot.org
