Anti-ATP10A antibody (440-690) (STJ118318)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP10A (440-690) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP10A |
| Gene ID | 57194 |
| Uniprot ID | AT10A_HUMAN |
| Immunogen Region | 440-690 |
| Immunogen Sequence | RRCTVSGVEYSHDANAQRLA RYQEADSEEEEVVPRGGSVS QRGSIGSHQSVRVVHRTQST KSHRRTGSRAEAKRASMLSK HTAFSSPMEKDITPDPKLLE KVSECDKSLAVARHQEHLLA HLSPELSDVFDFFIALTICN TVVVTSPDQPRTKVRVRFEL KSPVKTIEDFLRRFTPSCLT SGCSSIGSLAANKSSHKLGS SFPSTPSSDGMLLRLEERLG QPTSAIASNGYSSQADNWA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 440-690 of human ATP10A (NP_077816.1). |
Additional Info
| Tissue Specificity | Widely expressed, with highest levels in kidney, followed by lung, brain, prostate, testis, ovary and small intestine. |
| Post Translational Modifications | Autophosphorylated at the conserved aspartate of the P-type ATPase signature sequence. |
| Function | Catalytic component of P4-ATPase flippase complex, which catalyzes the hydrolysis of ATP coupled to the transport of phosphatidylcholine (PC) from the outer to the inner leaflet of the plasma membrane. Initiates inward plasma membrane bending and recruitment of Bin/amphiphysin/Rvs (BAR) domain-containing proteins involved in membrane tubulation and cell trafficking. Facilitates ITGB1/beta1 integrin endocytosis, delaying cell adhesion and cell spreading on extracellular matrix. Has low flippase activity toward glucosylceramide (GlcCer). |
| Protein Name | Phospholipid-Transporting Atpase VaAtpase Class V Type 10aAminophospholipid Translocase VaP4-Atpase Flippase Complex Alpha Subunit Atp10a |
| Database Links | Reactome: R-HSA-936837 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndoplasmic Reticulum MembraneExit From The Endoplasmic Reticulum Requires The Presence Of Tmem30aBut Not That Of Tmem30b |
| Alternative Antibody Names | Anti-Phospholipid-Transporting Atpase Va antibodyAnti-Atpase Class V Type 10a antibodyAnti-Aminophospholipid Translocase Va antibodyAnti-P4-Atpase Flippase Complex Alpha Subunit Atp10a antibodyAnti-ATP10A antibodyAnti-ATP10C antibodyAnti-ATPVA antibodyAnti-ATPVC antibodyAnti-KIAA0566 antibody |
Information sourced from Uniprot.org