Anti-ATL2 antibody (390-470) (STJ118329)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATL2 (390-470) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATL2 |
| Gene ID | 64225 |
| Uniprot ID | ATLA2_HUMAN |
| Immunogen Region | 390-470 |
| Immunogen Sequence | ARDTYCKSMEQVCGGDKPYI APSDLERKHLDLKEVAIKQF RSVKKMGGDEFCRRYQDQLE AEIEETYANFIKHNDGKNIF Y |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 390-470 of human ATL2 (NP_001129145.1). |
Additional Info
| Tissue Specificity | Expressed in peripheral tissues (at protein level). |
| Function | Atlastin-2 (ATL2) is a membrane-anchored GTPase that mediates the GTP-dependent fusion of endoplasmic reticulum (ER) membranes, maintaining the continuous ER network. It facilitates the formation of three-way junctions where ER tubules intersect. Two atlastin-2 on neighboring ER tubules bind GTP and form loose homodimers through the GB1/RHD3-type G domains and 3HB regions. Upon GTP hydrolysis, the 3HB regions tighten, pulling the membranes together to drive their fusion. After fusion, the homodimer disassembles upon release of inorganic phosphate (Pi). Subsequently, GDP dissociates, resetting the monomers to a conformation ready for a new fusion cycle. |
| Protein Name | Atlastin-2Atl-2Adp-Ribosylation Factor-Like Protein 6-Interacting Protein 2 |
| Database Links | |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinLocalizes At Endoplasmic Reticulum (Er) Three-Way Tubular Junctions |
| Alternative Antibody Names | Anti-Atlastin-2 antibodyAnti-Atl-2 antibodyAnti-Adp-Ribosylation Factor-Like Protein 6-Interacting Protein 2 antibodyAnti-ATL2 antibodyAnti-ARL6IP2 antibody |
Information sourced from Uniprot.org