• Western blot analysis of extracts of HUH-7 cells, using ATG9B antibody (STJ29543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using ATG9B antibody (STJ29543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of human placenta cells using ATG9B rabbit polyclonal antibody (STJ29543) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG9B antibody (715-924) (STJ29543)

SKU:
STJ29543

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATG9B (715-924) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG9B
Gene ID: 285973
Uniprot ID: ATG9B_HUMAN
Immunogen Region: 715-924
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 715-924 of human ATG9B (NP_001303985.1).
Immunogen Sequence: HPLWRPPGHSSKFLGHLWGR VQQDAAAWGATSARGPSTPG VLSNCTSPLPEAFLANLFVH PLLPPRDLSPTAPCPAAATA SLLASISRIAQDPSSVSPGG TGGQKLAQLPELASAEMSLH VIYLHQLHQQQQQQEPWGEA AASILSRPCSSPSQPPSPDE EKPSWSSDGSSPASSPRQQW GTQKARNLFPGGFQVTTDTQ KEPDRASCTD
Tissue Specificity Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial.
Function Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. In addition to autophagy, also plays a role in necrotic cell death.
Protein Name Autophagy-Related Protein 9b
Apg9-Like 2
Nitric Oxide Synthase 3-Overlapping Antisense Gene Protein
Protein Sone
Database Links Reactome: R-HSA-1632852
Cellular Localisation Preautophagosomal Structure Membrane
Multi-Pass Membrane Protein
Under Amino Acid Starvation Or Rapamycin Treatment
Redistributes From A Juxtanuclear Clustered Pool To A Dispersed Peripheral Cytosolic Pool
The Starvation-Induced Redistribution Depends On Ulk1 And Atg13
Alternative Antibody Names Anti-Autophagy-Related Protein 9b antibody
Anti-Apg9-Like 2 antibody
Anti-Nitric Oxide Synthase 3-Overlapping Antisense Gene Protein antibody
Anti-Protein Sone antibody
Anti-ATG9B antibody
Anti-APG9L2 antibody
Anti-NOS3AS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance