| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Cysteine Protease Atg4d (1-230) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATG4D |
| Gene ID: |
84971 |
| Uniprot ID: |
ATG4D_HUMAN |
| Immunogen Region: |
1-230 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ATG4D (NP_116274.3). |
| Immunogen Sequence: |
MNSVSPAAAQYRSSSPEDAR RRPEARRPRGPRGPDPNGLG PSGASGPALGSPGAGPSEPD EVDKFKAKFLTAWNNVKYGW VVKSRTSFSKISSIHLCGRR YRFEGEGDIQRFQRDFVSRL WLTYRRDFPPLPGGCLTSDC GWGCMLRSGQMMLAQGLLLH FLPRDWTWAEGMGLGPPELS GSASPSRYHGPARWMPPRWA QGAPELEQERRHRQIVSWFA DHPRAPFGLH |
| Tissue Specificity | Widely expressed in testis. |
| Post Translational Modifications | Cleaved by CASP3 during apoptosis which leads to increased activity. The cleavage by CASP3 reveals a cryptic mitochondrial targeting sequence immediately downstream of their canonical caspase cleavage sites which leads to mitochondrial import of the protein. |
| Function | Cysteine protease ATG4D: Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, by catalyzing delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). ATG4D plays a role in the autophagy-mediated neuronal homeostasis in the central nervous system. Compared to other members of the family (ATG4A, ATG4B or ATG4C), constitutes the major protein for the delipidation activity, while it promotes weak proteolytic activation of ATG8 proteins. Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy. Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability. |
| Protein Name | Cysteine Protease Atg4dAut-Like 4 Cysteine EndopeptidaseAutophagy-Related Cysteine Endopeptidase 4Autophagin-4Autophagy-Related Protein 4 Homolog DHsapg4d Cleaved Into - Cysteine Protease Atg4d - Mitochondrial |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Cysteine Protease Atg4d: CytoplasmCysteine Protease Atg4dMitochondrial: CytoplasmMitochondrion MatrixImported Into Mitochondrial Matrix After Cleavage By Casp3 During Oxidative Stress And Cell Death |
| Alternative Antibody Names | Anti-Cysteine Protease Atg4d antibodyAnti-Aut-Like 4 Cysteine Endopeptidase antibodyAnti-Autophagy-Related Cysteine Endopeptidase 4 antibodyAnti-Autophagin-4 antibodyAnti-Autophagy-Related Protein 4 Homolog D antibodyAnti-Hsapg4d Cleaved Into - Cysteine Protease Atg4d - Mitochondrial antibodyAnti-ATG4D antibodyAnti-APG4D antibodyAnti-AUTL4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ATG4D knockout (KO) HeLa cells, using [KO Validated] ATG4D Rabbit polyclonal antibody (STJ11100070) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89140/357590/STJ11100070_1__34146.1713121727.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ATG4D knockout (KO) HeLa cells, using [KO Validated] ATG4D Rabbit polyclonal antibody (STJ11100070) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
