| Host: |
Rabbit |
| Applications: |
IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Cysteine protease ATG4B is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC-P 1:50-300IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATG4B |
| Gene ID: |
23192 |
| Uniprot ID: |
ATG4B_HUMAN |
| Specificity: |
The antibody detects endogenous ATG4b protein |
| Immunogen: |
Recombinant Protein of ATG4b |
| Function | Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, by catalyzing delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Compared to other members of the family (ATG4A, ATG4C or ATG4C), constitutes the major protein for proteolytic activation of ATG8 proteins, while it displays weaker delipidation activity than other ATG4 paralogs. Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy. |
| Protein Name | Cysteine Protease Atg4bAut-Like 1 Cysteine EndopeptidaseAutophagy-Related Cysteine Endopeptidase 1Autophagin-1Autophagy-Related Protein 4 Homolog BHsapg4bHapg4b |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | CytoplasmCytosolCytoplasmic VesicleAutophagosomeEndoplasmic ReticulumMitochondrionMainly Localizes To The CytoplasmIncluding CytosolA Samll Potion Localizes To MitochondriaPhosphorylation At Ser-34 Promotes Localization To Mitochondria |
| Alternative Antibody Names | Anti-Cysteine Protease Atg4b antibodyAnti-Aut-Like 1 Cysteine Endopeptidase antibodyAnti-Autophagy-Related Cysteine Endopeptidase 1 antibodyAnti-Autophagin-1 antibodyAnti-Autophagy-Related Protein 4 Homolog B antibodyAnti-Hsapg4b antibodyAnti-Hapg4b antibodyAnti-ATG4B antibodyAnti-APG4B antibodyAnti-AUTL1 antibodyAnti-KIAA0943 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
