| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ATF7 (1-117) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATF7 |
| Gene ID: |
11016 |
| Uniprot ID: |
ATF7_HUMAN |
| Immunogen Region: |
1-117 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human ATF7 (NP_001193611.1). |
| Immunogen Sequence: |
MGDDRPFVCNAPGCGQRFTN EDHLAVHKHKHEMTLKFGPA RTDSVIIADQTPTPTRFLKN CEEVGLFNELASSFEHEFKK AADEDEKKARSRTVAKKLVV FRPRLFLLCFGIIFLIG |
| Tissue Specificity | Expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta. Isoform 4: Strongly expressed in skeletal muscle. Also expressed at lower levels in heart and lung. |
| Post Translational Modifications | On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity. Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase. On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity. Social isolation stress as well as TNF-alpha also induce the phosphorylation of ATF7. Phosphorylated in proliferating colonic and small intestinal epithelial cells. |
| Function | Stress-responsive chromatin regulator that plays a role in various biological processes including innate immunological memory, adipocyte differentiation or telomerase regulation. In absence of stress, contributes to the formation of heterochromatin and heterochromatin-like structure by recruiting histone H3K9 tri- and di-methyltransferases thus silencing the transcription of target genes such as STAT1 in adipocytes, or genes involved in innate immunity in macrophages and adipocytes. Stress induces ATF7 phosphorylation that disrupts interactions with histone methyltransferase and enhances the association with coactivators containing histone acetyltransferase and/or histone demethylase, leading to disruption of the heterochromatin-like structure and subsequently transcriptional activation. In response to TNF-alpha, which is induced by various stresses, phosphorylated ATF7 and telomerase are released from telomeres leading to telomere shortening. Also plays a role in maintaining epithelial regenerative capacity and protecting against cell death during intestinal epithelial damage and repair. Isoform 4: Acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter. Isoform 5: Acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation. |
| Protein Name | Cyclic Amp-Dependent Transcription Factor Atf-7Camp-Dependent Transcription Factor Atf-7Activating Transcription Factor 7Transcription Factor Atf-A |
| Cellular Localisation | NucleusNucleoplasmChromosomeTelomereMainly NucleoplasmicRestricted Distribution To The Perinuculear RegionThe Sumoylated Form Locates To The Nuclear PeripheryIsoform 5: Cytoplasm |
| Alternative Antibody Names | Anti-Cyclic Amp-Dependent Transcription Factor Atf-7 antibodyAnti-Camp-Dependent Transcription Factor Atf-7 antibodyAnti-Activating Transcription Factor 7 antibodyAnti-Transcription Factor Atf-A antibodyAnti-ATF7 antibodyAnti-ATFA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
