Anti-ATAD5 antibody (1054-1104 aa) (STJ195862)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human ATAD5 at the amino acid range 1054-1104
General Information
| Short Description | Rabbit polyclonal anti-ATPase family AAA domain-containing protein 5 (1054-1104 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATAD5 |
| Gene ID | 79915 |
| Uniprot ID | ATAD5_HUMAN |
| Immunogen | Synthesized peptide derived from the human ATAD5 at the amino acid range 1054-1104 |
| Immunogen Region | 1054-1104 aa |
| Specificity | This antibody detects endogenous levels of ATAD5 at Human/Mouse |
Additional Info
| Function | Has an important role in DNA replication and in maintaining genome integrity during replication stress. Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damage-induced apoptosis. Promotes PCNA deubiquitination by recruiting the ubiquitin-specific protease 1 (USP1) and WDR48 thereby down-regulating the error-prone damage bypass pathway. As component of the ATAD5 RFC-like complex, regulates the function of the DNA polymerase processivity factor PCNA by unloading the ring-shaped PCNA homotrimer from DNA after replication during the S phase of the cell cycle. This seems to be dependent on its ATPase activity. Plays important roles in restarting stalled replication forks under replication stress, by unloading the PCNA homotrimer from DNA and recruiting RAD51 possibly through an ATR-dependent manner. Ultimately this enables replication fork regression, breakage, and eventual fork restart. Both the PCNA unloading activity and the interaction with WDR48 are required to efficiently recruit RAD51 to stalled replication forks. Promotes the generation of MUS81-mediated single-stranded DNA-associated breaks in response to replication stress, which is an alternative pathway to restart stalled/regressed replication forks. |
| Protein Name | Atpase Family Aaa Domain-Containing Protein 5Chromosome Fragility-Associated Gene 1 Protein |
| Database Links | |
| Cellular Localisation | NucleusAccumulates In Nuclear Foci At Sites Of Stalled Dna Replication Forks In Response To Dna Damage |
| Alternative Antibody Names | Anti-Atpase Family Aaa Domain-Containing Protein 5 antibodyAnti-Chromosome Fragility-Associated Gene 1 Protein antibodyAnti-ATAD5 antibodyAnti-C17orf41 antibodyAnti-Elg1 antibodyAnti-FRAG1 antibody |
Information sourced from Uniprot.org