Confocal imaging ofย paraffin-embedded Mouse colon tissue usingย ASH2L Rabbit monoclonal antibody (STJ11102184, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-ASH2L antibody (529-628) [S4MR] (STJ11102184)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102184
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General Information

Short DescriptionRabbit monoclonal ASH2L (529-628) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS4MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolASH2L
Gene ID9070
Uniprot IDASH2L_HUMAN
Immunogen Region529-628
Immunogen SequenceAEKSLKQTPHSEIIFYKNGV NQGVAYKDIFEGVYFPAISL YKSCTVSINFGPCFKYPPKD LTYRPMSDMGWGAVVEHTLA DVLYHVETEVDGRRSPPWEP
SpecificityA synthetic peptide corresponding to a sequence within amino acids 529-628 of human ASH2L (Q9UBL3).

Additional Info

Tissue Specificity Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.
Post Translational Modifications Both monomethylated and dimethylated on arginine residues in the C-terminus. Arg-296 is the major site. Methylation is not required for nuclear localization, nor for MLL complex integrity or maintenance of global histone H3K4me3 levels.
Function Transcriptional regulator. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May play a role in hematopoiesis. In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
Protein Name Set1/Ash2 Histone Methyltransferase Complex Subunit Ash2
Ash2-Like Protein
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-3214841
Reactome: R-HSA-3769402
Reactome: R-HSA-5617472
Reactome: R-HSA-8936459
Reactome: R-HSA-9772755
Reactome: R-HSA-9818564
Reactome: R-HSA-9841922
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Set1/Ash2 Histone Methyltransferase Complex Subunit Ash2 antibody
Anti-Ash2-Like Protein antibody
Anti-ASH2L antibody
Anti-ASH2L1 antibody

Information sourced from Uniprot.org

Citations

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