• Immunohistochemistry analysis of paraffin-embedded human breast cancer using ASCL4 antibody (STJ116649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using ASCL4 antibody (STJ116649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using ASCL4 antibody (STJ116649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ASCL4 antibody ( STJ116649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-ASCL4 antibody (1-70) (STJ116649)

SKU:
STJ116649

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASCL4 (1-70) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASCL4
Gene ID: 121549
Uniprot ID: ASCL4_HUMAN
Immunogen Region: 1-70
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human ASCL4 (NP_982260.3).
Immunogen Sequence: METRKPAERLALPYSLRTAP LGVPGTLPGLPRRDPLRVAL RLDAACWEWARSGCARGWQY LPVPLDSAFE
Tissue Specificity Expressed in skin. 7-fold higher expression in fetal skin than in adult skin. Weak expression also detected in fetal lung, aorta and brain, and in adult stomach, kidney, ovary and breast.
Function Could be a transcriptional regulator involved in skin development.
Protein Name Achaete-Scute Homolog 4
Ash-4
Hash4
Achaete-Scute-Like Protein 4
Class A Basic Helix-Loop-Helix Protein 44
Bhlha44
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Achaete-Scute Homolog 4 antibody
Anti-Ash-4 antibody
Anti-Hash4 antibody
Anti-Achaete-Scute-Like Protein 4 antibody
Anti-Class A Basic Helix-Loop-Helix Protein 44 antibody
Anti-Bhlha44 antibody
Anti-ASCL4 antibody
Anti-BHLHA44 antibody
Anti-HASH4 antibody

Information sourced from Uniprot.org

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