Anti-ASCC2 antibody (488-757) (STJ115734)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ASCC2 (488-757) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ASCC2 |
| Gene ID | 84164 |
| Uniprot ID | ASCC2_HUMAN |
| Immunogen Region | 488-757 |
| Immunogen Sequence | LACLEYYHYDPEQVINNILE ERLAPTLSQLDRNLDREMKP DPTPLLTSRHNVFQNDEFDV FSRDSVDLSRVHKGKSTRKE ENTRSLLNDKRAVAAQRQRY EQYSVVVEEVPLQPGESLPY HSVYYEDEYDDTYDGNQVGA NDADSDDELISRRPFTIPQV LRTKVPREGQEEDDDDEEDD ADEEAPKPDHFVQDPAVLRE KAEARRMAFLAKKGYRHDSS TAVAGSPRGHGQSRETTQE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 488-757 of human ASCC2 (NP_115580.2). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | Ubiquitin-binding protein involved in DNA repair and rescue of stalled ribosomes. Plays a role in DNA damage repair as component of the ASCC complex. Recruits ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation. Involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation. Specifically recognizes and binds RPS20/uS10 ubiquitinated by ZNF598, promoting recruitment of the RQT (ribosome quality control trigger) complex on stalled ribosomes, followed by disassembly of stalled ribosomes. |
| Protein Name | Activating Signal Cointegrator 1 Complex Subunit 2Asc-1 Complex Subunit P100Trip4 Complex Subunit P100 |
| Database Links | Reactome: R-HSA-112126 |
| Cellular Localisation | NucleusNucleus SpeckleColocalizes With The Spliceosomal Components Prpf8 And Snrnp200/Brr2 In Nuclear Foci When Cells Have Been Exposed To Alkylating Agents That Cause Dna DamageColocalizes With Rnf113a And 'Lys-63'-Linked Polyubiquitinated ProteinsAlkbh3 And Ascc3 In Nuclear Foci When Cells Have Been Exposed To Alkylating Agents That Cause Dna Damage |
| Alternative Antibody Names | Anti-Activating Signal Cointegrator 1 Complex Subunit 2 antibodyAnti-Asc-1 Complex Subunit P100 antibodyAnti-Trip4 Complex Subunit P100 antibodyAnti-ASCC2 antibodyAnti-ASC1P100 antibodyAnti-RQT3 antibody |
Information sourced from Uniprot.org