| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ASAH1 (140-389) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ASAH1 |
| Gene ID: |
427 |
| Uniprot ID: |
ASAH1_HUMAN |
| Immunogen Region: |
140-389 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 140-389 of human ASAH1 (NP_001120977.1). |
| Immunogen Sequence: |
IVAEDKKGHLIHGRNMDFGV FLGWNINNDTWVITEQLKPL TVNLDFQRNNKTVFKASSFA GYVGMLTGFKPGLFSLTLNE RFSINGGYLGILEWILGKKD VMWIGFLTRTVLENSTSYEE AKNLLTKTKILAPAYFILGG NQSGEGCVITRDRKESLDVY ELDAKQGRWYVVQTNYDRWK HPFFLDDRRTPAKMCLNRTS QENISFETMYDVLSTKPVLN KLTVYTTLIDVTKGQFETY |
| Tissue Specificity | Broadly expressed with higher expression in heart. |
| Post Translational Modifications | N-glycosylated. Proteolytically cleaved into two chains alpha and beta that remain associated via a disulfide bond. Cleavage gives rise to a conformation change that activates the enzyme. The same catalytic Cys residue mediates the autoproteolytic cleavage and subsequent hydrolysis of lipid substrates. The beta chain may undergo an additional C-terminal processing. |
| Function | Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Has a higher catalytic efficiency towards C12-ceramides versus other ceramides. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used. Has also an N-acylethanolamine hydrolase activity. By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes. Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis. By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis. Isoform 2: May directly regulate steroidogenesis by binding the nuclear receptor NR5A1 and negatively regulating its transcriptional activity. |
| Protein Name | Acid CeramidaseAcAcdaseAcid CdaseAcylsphingosine DeacylaseN-Acylethanolamine Hydrolase Asah1N-Acylsphingosine AmidohydrolasePutative 32 Kda Heart ProteinPhp32 Cleaved Into - Acid Ceramidase Subunit Alpha - Acid Ceramidase Subunit Beta |
| Database Links | Reactome: R-HSA-1660662Reactome: R-HSA-6798695 |
| Cellular Localisation | LysosomeSecretedSecretion Is Extremely Low And Localization To Lysosomes Is Mannose-6-Phosphate Receptor-DependentIsoform 2: NucleusCytoplasmA Localization To The Nucleus And The Cytoplasm Has Also Been Reported For Asah1Most Probably For Isoforms Devoid Of A Signal Peptide |
| Alternative Antibody Names | Anti-Acid Ceramidase antibodyAnti-Ac antibodyAnti-Acdase antibodyAnti-Acid Cdase antibodyAnti-Acylsphingosine Deacylase antibodyAnti-N-Acylethanolamine Hydrolase Asah1 antibodyAnti-N-Acylsphingosine Amidohydrolase antibodyAnti-Putative 32 Kda Heart Protein antibodyAnti-Php32 Cleaved Into - Acid Ceramidase Subunit Alpha - Acid Ceramidase Subunit Beta antibodyAnti-ASAH1 antibodyAnti-ASAH antibodyAnti-HSD-33 antibodyAnti-HSD33 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
