| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Aromatase/CYP19A1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-0.1-0.3µg/mlIHC-4µg/mlELISA-antibody detection limit dilution 1:128000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
CYP19A1 |
| Gene ID: |
1588 |
| Uniprot ID: |
CP19A_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_000094.2; NP_112503.1 |
| Specificity: |
This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1) , which are identical |
| Immunogen Sequence: |
HDLSLHPDETKN |
| Post Translational Modifications | Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1). |
| Function | A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). |
| Protein Name | AromataseCypxixCytochrome P-450aromCytochrome P450 19a1Estrogen Synthase |
| Database Links | Reactome: R-HSA-193144Reactome: R-HSA-211976Reactome: R-HSA-5579030 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Aromatase antibodyAnti-Cypxix antibodyAnti-Cytochrome P-450arom antibodyAnti-Cytochrome P450 19a1 antibodyAnti-Estrogen Synthase antibodyAnti-CYP19A1 antibodyAnti-ARO1 antibodyAnti-CYAR antibodyAnti-CYP19 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
