| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:3000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ARL8B |
| Gene ID: | 55207 |
| Uniprot ID: | ARL8B_HUMAN |
| Immunogen Region: | 127-186 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 127-186 of human ARL8B (NP_060654.1). |
| Immunogen Sequence: | VLGNKRDLPNALDEKQLIEK MNLSAIQDREICCYSISCKE KDNIDITLQWLIQHSKSRRS |
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Ubiquitinated at Lys-141 by RNF167, leading to its degradation. |
| Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of lysosomal positioning which is important for nutrient sensing, natural killer cell-mediated cytotoxicity and antigen presentation. Along with its effectors, orchestrates lysosomal transport and fusion. Localizes specifically to lysosomal membranes and mediates anterograde lysosomal motility by recruiting PLEKHM2, which in turn recruits the motor protein kinesin-1 on lysosomes. Required for lysosomal and cytolytic granule exocytosis. Critical factor involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells. In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function. Also acts as a regulator of endosome to lysosome trafficking pathways of special significance for host defense. Recruits RUFY1 onto early endosomes regulating endosomes to trans-Golgi network proteins retrieval. Regulates cargo trafficking to lysosomes by binding to PLEKHM1 and recruiting the HOPS subunit VPS41, resulting in functional assembly of the HOPS complex on lysosomal membranes. Plays an important role in cargo delivery to lysosomes for antigen presentation and microbial killing. Directs the intersection of CD1d with lipid antigens in lysosomes, and plays a role in intersecting phagosomes with lysosomes to generate phagolysosomes that kill microbes. Involved in the process of MHC II presentation. Regulates the delivery of antigens to lysosomes and the formation of MHC II-peptide complexes through the recruitment of the HOPS complex to lysosomes allowing the fusion of late endosomes to lysosomes. May play a role in chromosome segregation. (Microbial infection) During Mycobacterium tuberculosis (Mtb) infection, is required for plasma membrane repair by controlling the exocytosis of lysosomes in macrophages. ARL8B secretion pathway is crucial to control the type of cell death of the M. tuberculosis-infected macrophages, distinguishing avirulent from virulent Mtb induced necrotic cell death. (Microbial infection) During infection, coronaviruses such as SARS-CoV-2 and the chaperone HSPA5/GRP78 are probably co-released through ARL8B-dependent lysosomal exocytic pathway for unconventional egress. |
| Protein Name | Adp-Ribosylation Factor-Like Protein 8b Adp-Ribosylation Factor-Like Protein 10c Novel Small G Protein Indispensable For Equal Chromosome Segregation 1 |
| Cellular Localisation | Late Endosome Membrane Lysosome Membrane Cytoplasm Cytoskeleton Spindle Cell Projection Axon Synapse Cytolytic Granule Membrane Early Endosome Membrane Gtp-Bound Form Resides On Lysosomal Membranes Whereas Gdp-Bound Form Is Likely Associated With Microtubular Structures Localizes With Microtubules At The Spindle Mid-Zone During Mitosis In Dendritic Cells Localizes To Mhc Ii+ Compartments |
| Alternative Antibody Names | Anti-Adp-Ribosylation Factor-Like Protein 8b antibody Anti-Adp-Ribosylation Factor-Like Protein 10c antibody Anti-Novel Small G Protein Indispensable For Equal Chromosome Segregation 1 antibody Anti-ARL8B antibody Anti-ARL10C antibody Anti-GIE1 antibody |
Information sourced from Uniprot.org