Anti-ARHGEF7 antibody (1-260) (STJ22679)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ARHGEF7 (1-260) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGEF7 |
| Gene ID | 8874 |
| Uniprot ID | ARHG7_HUMAN |
| Immunogen Region | 1-260 |
| Immunogen Sequence | MNSAEQTVTWLITLGVLESP KKTISDPEGFLQASLKDGVV LCRLLERLLPGTIEKTFDAN DLYQGQNFNKVLSSLVTLNK VTADIGLGSDSVCARPSSHR IKSFDSLGSQSLHTRTSKLF QGQYRSLDMTDNSNNQLVVR AKFNFQQTNEDELSFSKGDV IHVTRVEEGGWWEGTLNGRT GWFPSNYVREVKASEKPVSP KSGTLKSPPKGFDTTAINKS YYNVVLQNILETENEYSKE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ARHGEF7 (NP_001106984.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by PTK2/FAK1.this promotes interaction with RAC1. Phosphorylated on Ser-694 by CaMK1.enhancement of GEF activity and downstream activation of RAC1. |
| Function | Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 7Beta-PixCool-1Pak-Interacting Exchange Factor BetaP85 |
| Database Links | Reactome: R-HSA-182971Reactome: R-HSA-193648Reactome: R-HSA-3928664Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013406Reactome: R-HSA-9013409Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9619229 Q14155-1 |
| Cellular Localisation | Cell JunctionFocal AdhesionCell ProjectionRuffleCytoplasmCell CortexLamellipodiumDetected At Cell AdhesionsA Small Proportion Is Detected At Focal Adhesions |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 7 antibodyAnti-Beta-Pix antibodyAnti-Cool-1 antibodyAnti-Pak-Interacting Exchange Factor Beta antibodyAnti-P85 antibodyAnti-ARHGEF7 antibodyAnti-COOL1 antibodyAnti-KIAA0142 antibodyAnti-P85SPR antibodyAnti-PAK3BP antibodyAnti-PIXB antibodyAnti-Nbla10314 antibody |
Information sourced from Uniprot.org