Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4ยฐC overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ARHGAP31 antibody (730-810 aa) (STJ92189)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from CdGAP at the amino acid range 730-810
STJ92189
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General Information

Short DescriptionRabbit polyclonal anti-Rho GTPase-activating protein 31 (730-810 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/IF/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeIHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolARHGAP31
Gene ID57514
Uniprot IDRHG31_HUMAN
ImmunogenSynthesized peptide derived from CdGAP at the amino acid range 730-810
Immunogen Region730-810 aa
SpecificityCdGAP Polyclonal Antibody detects endogenous levels of CdGAP protein.

Additional Info

Function Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.
Protein Name Rho Gtpase-Activating Protein 31
Cdc42 Gtpase-Activating Protein
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013420
Cellular Localisation Cell Projection
Lamellipodium
Cell Junction
Focal Adhesion
Alternative Antibody Names Anti-Rho Gtpase-Activating Protein 31 antibody
Anti-Cdc42 Gtpase-Activating Protein antibody
Anti-ARHGAP31 antibody
Anti-CDGAP antibody
Anti-KIAA1204 antibody

Information sourced from Uniprot.org

Citations

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