• Immunofluorescence analysis of NIH/3T3 cells using ARHGAP30 Rabbit polyclonal antibody (STJ11102937) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ARHGAP30 Rabbit polyclonal antibody (STJ11102937) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse spleen, using ARHGAP30 Rabbit polyclonal antibody (STJ11102937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARHGAP30 antibody (485-585) (STJ11102937)

SKU:
STJ11102937

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGAP30
Gene ID: 257106
Uniprot ID: RHG30_HUMAN
Immunogen Region: 485-585
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 485-585 of human ARHGAP30 (NP_859071.2).
Immunogen Sequence: PLADSGPDDLAPALEDSLSQ EVQDSFSFLEDSSSSEPEWV GAEDGEVAQAEAAGAAFSPG EDDPGMGYLEELLGVGPQVE EFSVEPPLDDLSLDEAQFVL A
Function GTPase-activating protein (GAP) for RAC1 and RHOA, but not for CDC42.
Protein Name Rho Gtpase-Activating Protein 30
Rho-Type Gtpase-Activating Protein 30
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013420
Cellular Localisation Cytoplasmic Vesicle
Alternative Antibody Names Anti-Rho Gtpase-Activating Protein 30 antibody
Anti-Rho-Type Gtpase-Activating Protein 30 antibody
Anti-ARHGAP30 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance