• Immunofluorescence analysis of A549 cells, using ARFIP1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from Jurkat cells, using ARFIP1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARFIP1 antibody (271-320 aa) (STJ91675)

SKU:
STJ91675

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Arfaptin-1 (271-320 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARFIP1
Gene ID: 27236
Uniprot ID: ARFP1_HUMAN
Immunogen Region: 271-320 aa
Specificity: Arfaptin 1 Polyclonal Antibody detects endogenous levels of Arfaptin 1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ARFIP1 at the amino acid range 271-320
Post Translational Modifications Phosphorylated by PRKD1.phosphorylation delocalizes ARFIP1 from the Golgi and disrupts its ability to inhibit the activity of ADP-ribosylation factor, an important component of the vesicle scission machinery.
Function Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells.
Protein Name Arfaptin-1
Adp-Ribosylation Factor-Interacting Protein 1
Cellular Localisation Golgi Apparatus
Trans-Golgi Network Membrane
Alternative Antibody Names Anti-Arfaptin-1 antibody
Anti-Adp-Ribosylation Factor-Interacting Protein 1 antibody
Anti-ARFIP1 antibody

Information sourced from Uniprot.org

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