• Immunofluorescence analysis of U-2 OS cells using ARFGAP2 Rabbit polyclonal antibody (STJ119821) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ARFGAP2 Rabbit polyclonal antibody (STJ119821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of lysates from THP-1 cells, using ARFGAP2 Rabbit polyclonal antibody (STJ119821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-ARFGAP2 antibody (242-521) (STJ119821)

SKU:
STJ119821

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARFGAP2 (242-521) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARFGAP2
Gene ID: 84364
Uniprot ID: ARFG2_HUMAN
Immunogen Region: 242-521
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 242-521 of human ARFGAP2 (NP_115765.2).
Immunogen Sequence: SEIERQAQVAEKLREQQAAD AKKQAEESMVASMRLAYQEL QIDRKKEEKKLQNLEGKKRE QAERLGMGLVSRSSVSHSVL SEMQVIEQETPVSAKSSRSQ LDLFDDVGTFASGPPKYKDN PFSLGESFGSRWDTDAAWGM DRVEEKEPEVTISSIRPISE RATNRREVESRSSGLESSEA RQKFAGAKAISSDMFFGREV DAEYEARSRLQQLSGSSAIS SSDLFGDMDGAHGAGSVSL
Function GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
Protein Name Adp-Ribosylation Factor Gtpase-Activating Protein 2
Arf Gap 2
Gtpase-Activating Protein Znf289
Zinc Finger Protein 289
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-9013148
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Also Found On Peripheral Punctate Structures Likely To Be Endoplasmic Reticulum-Golgi Intermediate Compartment
Alternative Antibody Names Anti-Adp-Ribosylation Factor Gtpase-Activating Protein 2 antibody
Anti-Arf Gap 2 antibody
Anti-Gtpase-Activating Protein Znf289 antibody
Anti-Zinc Finger Protein 289 antibody
Anti-ARFGAP2 antibody
Anti-ZNF289 antibody
Anti-Nbla10535 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance