Confocal imaging of NIH/3T3 cells usingย ARF5 Rabbit monoclonal antibody (STJ11101858, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-ARF5 antibody (81-180) [S8MR] (STJ11101858)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101858
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General Information

Short DescriptionRabbit monoclonal ARF5 (81-180) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS8MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolARF5
Gene ID381
Uniprot IDARF5_HUMAN
Immunogen Region81-180
Immunogen SequenceYFQNTQGLIFVVDSNDRERV QESADELQKMLQEDELRDAV LLVFANKQDMPNAMPVSELT DKLGLQHLRSRTWYVQATCA TQGTGLYDGLDWLSHELSKR
SpecificityA synthetic peptide corresponding to a sequence within amino acids 81-180 of human ARF5 (P84085).

Additional Info

Function GTP-binding protein involved in protein trafficking.may modulate vesicle budding and uncoating within the Golgi apparatus. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Protein Name Adp-Ribosylation Factor 5
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Golgi Apparatus
Cytoplasm
Perinuclear Region
Membrane
Lipid-Anchor
Trans-Golgi Network Membrane
Alternative Antibody Names Anti-Adp-Ribosylation Factor 5 antibody
Anti-ARF5 antibody

Information sourced from Uniprot.org

Citations

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