• Confocal imaging of NIH/3T3 cells using ARF5 Rabbit monoclonal antibody (STJ11101858, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using ARF5 Rabbit monoclonal antibody (STJ11101858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ARF5 antibody (81-180) [S8MR] (STJ11101858)

SKU:
STJ11101858

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ARF5 (81-180) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARF5
Gene ID: 381
Uniprot ID: ARF5_HUMAN
Immunogen Region: 81-180
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 81-180 of human ARF5 (P84085).
Immunogen Sequence: YFQNTQGLIFVVDSNDRERV QESADELQKMLQEDELRDAV LLVFANKQDMPNAMPVSELT DKLGLQHLRSRTWYVQATCA TQGTGLYDGLDWLSHELSKR
Function GTP-binding protein involved in protein trafficking.may modulate vesicle budding and uncoating within the Golgi apparatus. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Protein Name Adp-Ribosylation Factor 5
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Golgi Apparatus
Cytoplasm
Perinuclear Region
Membrane
Lipid-Anchor
Trans-Golgi Network Membrane
Alternative Antibody Names Anti-Adp-Ribosylation Factor 5 antibody
Anti-ARF5 antibody

Information sourced from Uniprot.org

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