• Immunofluorescence analysis of mouse kidney cells using AQP2 antibody (STJ118649) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat kidney cells using AQP2 antibody (STJ118649) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using AQP2 antibody (STJ118649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of 293T cells, using AQP2 antibody (STJ118649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AQP2 antibody (147-271) (STJ118649)

SKU:
STJ118649

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AQP2 (147-271) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP2
Gene ID: 359
Uniprot ID: AQP2_HUMAN
Immunogen Region: 147-271
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 147-271 of human AQP2 (NP_000477.1).
Immunogen Sequence: ASTDERRGENPGTPALSIGF SVALGHLLGIHYTGCSMNPA RSLAPAVVTGKFDDHWVFWI GPLVGAILGSLLYNYVLFPP AKSLSERLAVLKGLEPDTDW EEREVRRRQSVELHSPQSLP RGTKA
Tissue Specificity Expressed in collecting tubules in kidney medulla (at protein level). Detected in kidney.
Post Translational Modifications Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. N-glycosylated.
Function Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis.
Protein Name Aquaporin-2
Aqp-2
Adh Water Channel
Aquaporin-Cd
Aqp-Cd
Collecting Duct Water Channel Protein
Wch-Cd
Water Channel Protein For Renal Collecting Duct
Database Links Reactome: R-HSA-432040
Reactome: R-HSA-432047
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Cell Membrane
Cytoplasmic Vesicle Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Shuttles From Vesicles To The Apical Membrane
Vasopressin-Regulated Phosphorylation Is Required For Translocation To The Apical Cell Membrane
Plekha8/Fapp2 Is Required To Transport Aqp2 From The Tgn To Sites Where Aqp2 Is Phosphorylated
Alternative Antibody Names Anti-Aquaporin-2 antibody
Anti-Aqp-2 antibody
Anti-Adh Water Channel antibody
Anti-Aquaporin-Cd antibody
Anti-Aqp-Cd antibody
Anti-Collecting Duct Water Channel Protein antibody
Anti-Wch-Cd antibody
Anti-Water Channel Protein For Renal Collecting Duct antibody
Anti-AQP2 antibody

Information sourced from Uniprot.org

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