Anti-AQP2 antibody (147-271) (STJ11103836)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Aquaporin-2 (147-271) antibody for use in IHC-P and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AQP2 |
| Gene ID | 359 |
| Uniprot ID | AQP2_HUMAN |
| Immunogen Region | 147-271 |
| Immunogen Sequence | ASTDERRGENPGTPALSIGF SVALGHLLGIHYTGCSMNPA RSLAPAVVTGKFDDHWVFWI GPLVGAILGSLLYNYVLFPP AKSLSERLAVLKGLEPDTDW EEREVRRRQSVELHSPQSLP RGTKA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 147-271 of human AQP2 (NP_000477.1). |
Additional Info
| Tissue Specificity | Expressed in collecting tubules in kidney medulla (at protein level). Detected in kidney. |
| Post Translational Modifications | Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. N-glycosylated. |
| Function | Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis. Could also be permeable to glycerol. |
| Protein Name | Aquaporin-2Aqp-2Adh Water ChannelAquaporin-CdAqp-CdCollecting Duct Water Channel ProteinWch-CdWater Channel Protein For Renal Collecting Duct |
| Database Links | Reactome: R-HSA-432040Reactome: R-HSA-432047 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneCell MembraneCytoplasmic Vesicle MembraneGolgi ApparatusTrans-Golgi Network MembraneShuttles From Vesicles To The Apical MembraneVasopressin-Regulated Phosphorylation Is Required For Translocation To The Apical Cell MembranePlekha8/Fapp2 Is Required To Transport Aqp2 From The Tgn To Sites Where Aqp2 Is Phosphorylated |
| Alternative Antibody Names | Anti-Aquaporin-2 antibodyAnti-Aqp-2 antibodyAnti-Adh Water Channel antibodyAnti-Aquaporin-Cd antibodyAnti-Aqp-Cd antibodyAnti-Collecting Duct Water Channel Protein antibodyAnti-Wch-Cd antibodyAnti-Water Channel Protein For Renal Collecting Duct antibodyAnti-AQP2 antibody |
Information sourced from Uniprot.org