Anti-AQP0 antibody (STJ13100019)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from human AQP0 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-AQP0 for use in IHC and WB in Human and Marmoset samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Marmoset |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:2000 to 1:3000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | MIP |
| Gene ID | 4284 |
| Uniprot ID | MIP_HUMAN |
| Immunogen | A synthetic peptide from human AQP0 conjugated to blue carrier protein was used as the antigen. |
| Specificity | Specific for AQP0. |
Additional Info
| Post Translational Modifications | Subject to partial proteolytic cleavage in the eye lens core. Partial proteolysis promotes interactions between tetramers from adjoining membranes. Fatty acylated at Met-1 and Lys-238. The acyl modifications, in decreasing order of ion abundance, are: oleoyl (C18:1) > palmitoyl (C16:0) > stearoyl (C18:0) > eicosenoyl (C20:1) > dihomo-gamma-linolenoyl (C20:3) > palmitoleoyl (C16:1) > eicosadienoyl (C20:2). |
| Function | Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Specifically expressed in lens fiber cells, this aquaporin is crucial for maintaining lens water homeostasis and transparency. Beyond water permeability, it also acts as a cell-to-cell adhesion molecule, forming thin junctions between lens fiber cells that are essential for maintaining the ordered structure and transparency of the lens. |
| Protein Name | Lens Fiber Major Intrinsic ProteinAquaporin-0Mip26Mp26 |
| Database Links | Reactome: R-HSA-432047 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell JunctionLocalizes To Thin Cell-Cell Junctions In Lens Fiber Cells |
| Alternative Antibody Names | Anti-Lens Fiber Major Intrinsic Protein antibodyAnti-Aquaporin-0 antibodyAnti-Mip26 antibodyAnti-Mp26 antibodyAnti-MIP antibodyAnti-AQP0 antibody |
Information sourced from Uniprot.org