• Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR7 Rabbit polyclonal antibody (STJ11104779) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-APRR7 antibody (236-339 aa) (STJ11104779)

SKU:
STJ11104779

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Host: Rabbit
Applications: WB/ELISA
Reactivity: A.thaliana
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 236-339 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 236-339 of Arabidopsis thaliana APRR7 (NP_568107.1).
Immunogen Sequence: ENENGSIGLNASDGSSDGSG AQSSWTKKAVDVDDSPRAVS LWDRVDSTCAQVVHSNPEFP SNQLVAPPAEKETQEHDDKF EDVTMGRDLEISIRRNCDLA LEPK
Background PRR7 and PRR9 are partially redundant essential components of a temperature-sensitive circadian system. CCA1 and LHY had a positive effect on PRR7 expression levels. Acts as transcriptional repressor of CCA1 and LHY.

Information sourced from Uniprot.org

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