Anti-APPL1 antibody (121-170 aa) (STJ91648)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human APPL1 at the amino acid range 121-170
General Information
| Short Description | Rabbit polyclonal anti-DCC-interacting protein 13-alpha (121-170 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | APPL1 |
| Gene ID | 26060 |
| Uniprot ID | DP13A_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human APPL1 at the amino acid range 121-170 |
| Immunogen Region | 121-170 aa |
| Specificity | APPL1 Polyclonal Antibody detects endogenous levels of APPL1 protein. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-410 by PKA severely impairs binding to OCRL. |
| Function | Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages. Regulates TLR4 signaling in activated macrophages. Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways. Required for fibroblast migration through HGF cell signaling. Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex. |
| Protein Name | Dcc-Interacting Protein 13-AlphaDip13-AlphaAdapter Protein Containing Ph Domain - Ptb Domain And Leucine Zipper Motif 1 |
| Database Links | Reactome: R-HSA-418889 |
| Cellular Localisation | Early Endosome MembranePeripheral Membrane ProteinNucleusCytoplasmEndosomeCell ProjectionRuffleCytoplasmic VesiclePhagosomeEarly Endosomal Membrane-Bound And NuclearTranslocated Into The Nucleus Upon Release From Endosomal Membranes Following Internalization Of Egf |
| Alternative Antibody Names | Anti-Dcc-Interacting Protein 13-Alpha antibodyAnti-Dip13-Alpha antibodyAnti-Adapter Protein Containing Ph Domain - Ptb Domain And Leucine Zipper Motif 1 antibodyAnti-APPL1 antibodyAnti-APPL antibodyAnti-DIP13A antibodyAnti-KIAA1428 antibody |
Information sourced from Uniprot.org