• Immunofluorescence analysis of L929 cells using ApoM Rabbit polyclonal antibody (STJ27289) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ApoM in paraffin-embedded Mouse kidney using ApoM Rabbit polyclonal antibody (STJ27289) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ApoM in paraffin-embedded Human colon using ApoM Rabbit polyclonal antibody (STJ27289) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ApoM Rabbit polyclonal antibody (STJ27289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-APOM antibody (22-188) (STJ27289)

SKU:
STJ27289

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOM (22-188) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOM
Gene ID: 55937
Uniprot ID: APOM_HUMAN
Immunogen Region: 22-188
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-188 of human ApoM (NP_061974.2).
Immunogen Sequence: QCPEHSQLTTLGVDGKEFPE VHLGQWYFIAGAAPTKEELA TFDPVDNIVFNMAAGSAPMQ LHLRATIRMKDGLCVPRKWI YHLTEGSTDLRTEGRPDMKT ELFSSSCPGGIMLNETGQGY QRFLLYNRSPHPPEKCVEEF KSLTSCLDSKAFLLTPRNQE ACELSNN
Tissue Specificity Plasma protein. Expressed in liver and kidney.
Function Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Protein Name Apolipoprotein M
Apo-M
Apom
Protein G3a
Database Links Reactome: R-HSA-975634
Cellular Localisation Secreted
Present In High Density Lipoprotein (Hdl) And To A Lesser Extent In Triglyceride-Rich Lipoproteins (Tgrlp) And Low Density Lipoproteins (Ldl)
Alternative Antibody Names Anti-Apolipoprotein M antibody
Anti-Apo-M antibody
Anti-Apom antibody
Anti-Protein G3a antibody
Anti-APOM antibody
Anti-G3A antibody
Anti-NG20 antibody
Anti-HSPC336 antibody

Information sourced from Uniprot.org

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