• Western blot analysis of extracts of various cell lines, using Apolipoprotein C3 antibody (STJ116430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using Apolipoprotein C3 antibody (STJ116430) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Apolipoprotein C3 antibody (STJ116430) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using Apolipoprotein C3 antibody (STJ116430) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HepG2 cells using Apolipoprotein C3 rabbit polyclonal antibody (STJ116430) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse liver cells using Apolipoprotein C3 rabbit polyclonal antibody (STJ116430) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat liver cells using Apolipoprotein C3 rabbit polyclonal antibody (STJ116430) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-APOC3 antibody (1-99) (STJ116430)

SKU:
STJ116430

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOC3 (1-99) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOC3
Gene ID: 345
Uniprot ID: APOC3_HUMAN
Immunogen Region: 1-99
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-99 of human Apolipoprotein C3 (NP_000031.1).
Immunogen Sequence: MQPRVLLVVALLALLASARA SEAEDASLLSFMQGYMKHAT KTAKDALSSVQESQVAQQAR GWVTDGFSSLKDYWSTVKDK FSEFWDLDPEVRPTSAVAA
Tissue Specificity Liver.
Post Translational Modifications The most abundant glycoforms are characterized by an O-linked disaccharide galactose linked to N-acetylgalactosamine (Gal-GalNAc), further modified with up to 3 sialic acid residues. Less abundant glycoforms are characterized by more complex and fucosylated glycan moieties. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
Function Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion.extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.
Protein Name Apolipoprotein C-Iii
Apo-Ciii
Apoc-Iii
Apolipoprotein C3
Database Links Reactome: R-HSA-8963888
Reactome: R-HSA-8963901
Reactome: R-HSA-8964058
Reactome: R-HSA-975634
Cellular Localisation Secreted
Alternative Antibody Names Anti-Apolipoprotein C-Iii antibody
Anti-Apo-Ciii antibody
Anti-Apoc-Iii antibody
Anti-Apolipoprotein C3 antibody
Anti-APOC3 antibody

Information sourced from Uniprot.org

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