Anti-APOA1 antibody (25-267) (STJ22644)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal APOA1 (25-267) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | APOA1 |
| Gene ID | 335 |
| Uniprot ID | APOA1_HUMAN |
| Immunogen Region | 25-267 |
| Immunogen Sequence | DEPPQSPWDRVKDLATVYVD VLKDSGRDYVSQFEGSALGK QLNLKLLDNWDSVTSTFSKL REQLGPVTQEFWDNLEKETE GLRQEMSKDLEEVKAKVQPY LDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEK LSPLGEEMRDRARAHVDALR THLAPYSDELRQRLAARLEA LKENGGARLAEYHAKATEHL STLSEKAKPALEDLRQGLLP VLESFKVSFLSALEEYTKK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1 (NP_000030.1). |
Additional Info
| Tissue Specificity | Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. |
| Post Translational Modifications | Glycosylated. Palmitoylated. Phosphorylation sites are present in the extracellular medium. |
| Function | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. |
| Protein Name | Apolipoprotein A-IApo-AiApoa-IApolipoprotein A1 Cleaved Into - Proapolipoprotein A-IProapoa-I - Truncated Apolipoprotein A-IApolipoprotein A-I(1-242 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1369062Reactome: R-HSA-1989781Reactome: R-HSA-2168880Reactome: R-HSA-3000471Reactome: R-HSA-3000480Reactome: R-HSA-381426Reactome: R-HSA-5682113Reactome: R-HSA-8957275Reactome: R-HSA-8963888Reactome: R-HSA-8963896Reactome: R-HSA-8963901Reactome: R-HSA-8964011Reactome: R-HSA-8964058Reactome: R-HSA-9707616Reactome: R-HSA-975634Reactome: R-HSA-977225 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Apolipoprotein A-I antibodyAnti-Apo-Ai antibodyAnti-Apoa-I antibodyAnti-Apolipoprotein A1 Cleaved Into - Proapolipoprotein A-I antibodyAnti-Proapoa-I - Truncated Apolipoprotein A-I antibodyAnti-Apolipoprotein A-I(1-242 antibodyAnti-APOA1 antibody |
Information sourced from Uniprot.org