• STJ72920 (0. 03µg/ml) staining of Human Frontal cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72920 (0. 1µg/ml) staining of Mouse Brain lysate (~5µg protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labelling. Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK. This data is from a previous batch, not on sale.
  • STJ72920 (0. 1µg/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK. This data is from a previous batch, not on sale.

Anti-AP2A1 antibody (706-727) (STJ72920)

SKU:
STJ72920-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-AP2A1 (706-727) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.03-0.1µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: AP2A1
Gene ID: 160
Uniprot ID: AP2A1_HUMAN
Immunogen Region: 706-727
Accession Number: NP_055018.2
Specificity: This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
Immunogen Sequence: ELEPPAPESPMALLADPAPAAD
Function Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-FILMV (Y-X-X-Phi) and ED-X-X-X-L-LI endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the ED-X-X-X-L-LI motif.
Protein Name Ap-2 Complex Subunit Alpha-1
100 Kda Coated Vesicle Protein A
Adaptor Protein Complex Ap-2 Subunit Alpha-1
Adaptor-Related Protein Complex 2 Subunit Alpha-1
Alpha-Adaptin A
Alpha1-Adaptin
Clathrin Assembly Protein Complex 2 Alpha-A Large Chain
Plasma Membrane Adaptor Ha2/Ap2 Adaptin Alpha A Subunit
Database Links Reactome: R-HSA-167590
Reactome: R-HSA-177504
Reactome: R-HSA-182218
Reactome: R-HSA-2132295
Reactome: R-HSA-3928665
Reactome: R-HSA-416993
Reactome: R-HSA-437239
Reactome: R-HSA-5099900
Reactome: R-HSA-5140745
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8866427
Reactome: R-HSA-8964038
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Membrane
Coated Pit
Peripheral Membrane Protein
Cytoplasmic Side
Ap-2 Appears To Be Excluded From Internalizing Ccvs And To Disengage From Sites Of Endocytosis Seconds Before Internalization Of The Nascent Ccv
Alternative Antibody Names Anti-Ap-2 Complex Subunit Alpha-1 antibody
Anti-100 Kda Coated Vesicle Protein A antibody
Anti-Adaptor Protein Complex Ap-2 Subunit Alpha-1 antibody
Anti-Adaptor-Related Protein Complex 2 Subunit Alpha-1 antibody
Anti-Alpha-Adaptin A antibody
Anti-Alpha1-Adaptin antibody
Anti-Clathrin Assembly Protein Complex 2 Alpha-A Large Chain antibody
Anti-Plasma Membrane Adaptor Ha2/Ap2 Adaptin Alpha A Subunit antibody
Anti-AP2A1 antibody
Anti-ADTAA antibody
Anti-CLAPA1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance