• Western blot analysis of extracts of various cell lines, using AP1M2 antibody (STJ110629) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 1s.
  • Immunofluorescence analysis of H9c2 cells using AP1M2 Rabbit polyclonal antibody (STJ110629) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of U2OS cells using AP1M2 Polyclonal Antibody (STJ110629) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using AP1M2 Rabbit polyclonal antibody (STJ110629) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using AP1M2 Rabbit polyclonal antibody (STJ110629) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of U2OS cells using AP1M2 Rabbit polyclonal antibody (STJ110629) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using AP1M2 Rabbit polyclonal antibody (STJ110629) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 1s.

Anti-AP1M2 antibody (164-423) (STJ110629)

SKU:
STJ110629

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AP1M2 (164-423) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AP1M2
Gene ID: 10053
Uniprot ID: AP1M2_HUMAN
Immunogen Region: 164-423
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 164-423 of human AP1M2 (NP_005489.2).
Immunogen Sequence: IKYKKNEVFIDVIESVNLLV NANGSVLLSEIVGTIKLKVF LSGMPELRLGLNDRVLFELT GRSKNKSVELEDVKFHQCVR LSRFDNDRTISFIPPDGDFE LMSYRLSTQVKPLIWIESVI EKFSHSRVEIMVKAKGQFKK QSVANGVEISVPVPSDADSP RFKTSVGSAKYVPERNVVIW SIKSFPGGKEYLMRAHFGLP SVEKEEVEGRPPIGVKFEIP YFTVSGIQVRYMKIIEKSG
Post Translational Modifications Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.
Function Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Protein Name Ap-1 Complex Subunit Mu-2
Ap-Mu Chain Family Member Mu1b
Adaptor Protein Complex Ap-1 Subunit Mu-2
Adaptor-Related Protein Complex 1 Subunit Mu-2
Clathrin Assembly Protein Complex 1 Mu-2 Medium Chain 2
Golgi Adaptor Ha1/Ap1 Adaptin Mu-2 Subunit
Mu-Adaptin 2
Mu1b-Adaptin
Database Links Reactome: R-HSA-164940
Reactome: R-HSA-2132295
Reactome: R-HSA-432720
Reactome: R-HSA-432722
Cellular Localisation Golgi Apparatus
Cytoplasmic Vesicle
Clathrin-Coated Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Component Of The Coat Surrounding The Cytoplasmic Face Of Coated Vesicles Located At The Golgi Complex
Alternative Antibody Names Anti-Ap-1 Complex Subunit Mu-2 antibody
Anti-Ap-Mu Chain Family Member Mu1b antibody
Anti-Adaptor Protein Complex Ap-1 Subunit Mu-2 antibody
Anti-Adaptor-Related Protein Complex 1 Subunit Mu-2 antibody
Anti-Clathrin Assembly Protein Complex 1 Mu-2 Medium Chain 2 antibody
Anti-Golgi Adaptor Ha1/Ap1 Adaptin Mu-2 Subunit antibody
Anti-Mu-Adaptin 2 antibody
Anti-Mu1b-Adaptin antibody
Anti-AP1M2 antibody

Information sourced from Uniprot.org

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