• Western blot analysis of lysates from HeLa cells, using c-Jun Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of HuvEc cells using AP-1/Jun D Polyclonal Antibody
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using c-Jun Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using AP-1/Jun D Polyclonal Antibody

Anti-AP-1/Jun D antibody (40-89 aa) (STJ91611)

SKU:
STJ91611

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transcription factor AP-1 and Transcription factor jun-D (40-89 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JUN
JUND
Gene ID: 3725
3727
Uniprot ID: JUN_HUMAN
JUND_HUMAN
Immunogen Region: 40-89 aa
Specificity: AP-1 Polyclonal Antibody detects endogenous levels of AP-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human c-Jun at the amino acid range 40-89
Post Translational Modifications Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7. Phosphorylated by CaMK4 and PRKDC.phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243.this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B.phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Acetylated at Lys-271 by EP300.
Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGACGTCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells.
Protein Name Transcription Factor Ap-1
Activator Protein 1
Ap1
Proto-Oncogene C-Jun
V-Jun Avian Sarcoma Virus 17 Oncogene Homolog
P39
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2871796
Reactome: R-HSA-450341
Reactome: R-HSA-5617472
Reactome: R-HSA-5687128
Reactome: R-HSA-6796648
Reactome: R-HSA-8862803
Reactome: R-HSA-8943724
Reactome: R-HSA-9018519
Reactome: R-HSA-9673324
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Ap-1 antibody
Anti-Activator Protein 1 antibody
Anti-Ap1 antibody
Anti-Proto-Oncogene C-Jun antibody
Anti-V-Jun Avian Sarcoma Virus 17 Oncogene Homolog antibody
Anti-P39 antibody
Anti-JUN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance