![Western blot analysis of lysates from wild type (WT) and Annexin A6/Annexin VI knockout (KO) HeLa cells, using [KO Validated] Annexin A6/Annexin VI Rabbit polyclonal antibody (STJ11100042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89114/357566/STJ11100042_1__16062.1713121692.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Annexin A6/Annexin VI knockout (KO) HeLa cells, using [KO Validated] Annexin A6/Annexin VI Rabbit polyclonal antibody (STJ11100042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and Annexin A6/Annexin VI knockout (KO) HeLa cells, using [KO Validated] Annexin A6/Annexin VI Rabbit polyclonal antibody (STJ11100042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89114/357566/STJ11100042_1__16062.1713121692.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Annexin A6/Annexin VI knockout (KO) HeLa cells, using [KO Validated] Annexin A6/Annexin VI Rabbit polyclonal antibody (STJ11100042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-ANXA6 antibody (1-250) (STJ11100042)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ANXA6 (1-250) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ANXA6 |
| Gene ID | 309 |
| Uniprot ID | ANXA6_HUMAN |
| Immunogen Region | 1-250 |
| Immunogen Sequence | MAKPAQGAKYRGSIHDFPGF DPNQDAEALYTAMKGFGSDK EAILDIITSRSNRQRQEVCQ SYKSLYGKDLIADLKYELTG KFERLIVGLMRPPAYCDAKE IKDAISGIGTDEKCLIEILA SRTNEQMHQLVAAYKDAYER DLEADIIGDTSGHFQKMLVV LLQGTREEDDVVSEDLVQQD VQDLYEAGELKWGTDEAQFI YILGNRSKQHLRLVFDEYLK TTGKPIEASIRGELSGDFE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Annexin A6/Annexin VI (NP_001146.2). |
Additional Info
| Post Translational Modifications | Phosphorylated in response to growth factor stimulation. |
| Function | May associate with CD21. May regulate the release of Ca(2+) from intracellular stores. |
| Protein Name | Annexin A667 Kda CalelectrinAnnexin ViAnnexin-6Calphobindin-IiCpb-IiChromobindin-20Lipocortin ViProtein IiiP68P70 |
| Database Links | Reactome: R-HSA-445355 |
| Cellular Localisation | CytoplasmMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Annexin A6 antibodyAnti-67 Kda Calelectrin antibodyAnti-Annexin Vi antibodyAnti-Annexin-6 antibodyAnti-Calphobindin-Ii antibodyAnti-Cpb-Ii antibodyAnti-Chromobindin-20 antibodyAnti-Lipocortin Vi antibodyAnti-Protein Iii antibodyAnti-P68 antibodyAnti-P70 antibodyAnti-ANXA6 antibodyAnti-ANX6 antibody |
Information sourced from Uniprot.org