• Immunohistochemistry analysis of paraffin-embedded mouse kidney using ANTXR2 antibody (STJ28609) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using ANTXR2 antibody (STJ28609) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ANTXR2 Rabbit polyclonal antibody (STJ28609) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ANTXR2 antibody (34-300) (STJ28609)

SKU:
STJ28609

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANTXR2 (34-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANTXR2
Gene ID: 118429
Uniprot ID: ANTR2_HUMAN
Immunogen Region: 34-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-300 of human ANTXR2 (NP_001139266.1).
Immunogen Sequence: QEQPSCRRAFDLYFVLDKSG SVANNWIEIYNFVQQLAERF VSPEMRLSFIVFSSQATIIL PLTGDRGKISKGLEDLKRVS PVGETYIHEGLKLANEQIQK AGGLKTSSIIIALTDGKLDG LVPSYAEKEAKISRSLGASV YCVGVLDFEQAQLERIADSK EQVFPVKGGFQALKGIINSI LAQSCTEILELQPSSVCVGE EFQIVLSGRGFMLGSRNGSV LCTYTVNETYTTSVKPVSV
Tissue Specificity Expressed in prostate, thymus, ovary, testis, pancreas, colon, heart, kidney, lung, liver, peripheral blood leukocytes, placenta, skeletal muscle, small intestine and spleen.
Function Necessary for cellular interactions with laminin and the extracellular matrix. (Microbial infection) Receptor for the protective antigen (PA) of B.anthracis. Binding of PA leads to heptamerization of the receptor-PA complex. Upon binding of the PA of B.anthracis, the complex moves to glycosphingolipid-rich lipid rafts, where it is internalized via a clathrin-dependent pathway. In the endosomal membrane, at pH under 7, the complex then rearranges and forms a pore allowing the other components of anthrax toxin to escape to the cytoplasm.
Protein Name Anthrax Toxin Receptor 2
Capillary Morphogenesis Gene 2 Protein
Cmg-2
Database Links Reactome: R-HSA-5210891
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Expressed At The Cell Surface
Isoform 2: Endoplasmic Reticulum Membrane
Expressed Predominantly Within The Endoplasmic Reticulum And Not At The Plasma Membrane
Isoform 3: Secreted
Alternative Antibody Names Anti-Anthrax Toxin Receptor 2 antibody
Anti-Capillary Morphogenesis Gene 2 Protein antibody
Anti-Cmg-2 antibody
Anti-ANTXR2 antibody
Anti-CMG2 antibody

Information sourced from Uniprot.org

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