• Western blot analysis of extracts of various cell lines, using ANKHD1 antibody (STJ110523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using ANKHD1 antibody (STJ110523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ANKHD1 antibody (1620-1800) (STJ110523)

SKU:
STJ110523

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANKHD1 (1620-1800) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANKHD1
Gene ID: 404734/54882
Uniprot ID: ANKH1_HUMAN
Immunogen Region: 1620-1800
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1620-1800 of human ANKHD1 (NP_060217.1).
Immunogen Sequence: VNSSKYPSLLLHSQEEKTST ATSKTQTRLEGEVTPNSLST SYKTVSLPLSSPNIKLNLTS PKRGQKREEGWKEVVRRSKK LSVPASVVSRIMGRGGCNIT AIQDVTGAHIDVDKQKDKNG ERMITIRGGTESTRYAVQLI NALIQDPAKELEDLIPKNHI RTPASTKSIHANFSSGVGTT A
Tissue Specificity Ubiquitous with high expression in cervix, spleen and brain. Expressed in hematopoietic cells with increased expression in leukemia cells. Isoform 2 is highly expressed in spleen with almost no expression in muscle and brain.
Function May play a role as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells. Isoform 2 may possess an antiapoptotic effect and protect cells during normal cell survival through its regulation of caspases.
Protein Name Ankyrin Repeat And Kh Domain-Containing Protein 1
Hiv-1 Vpr-Binding Ankyrin Repeat Protein
Multiple Ankyrin Repeats Single Kh Domain
Hmask
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ankyrin Repeat And Kh Domain-Containing Protein 1 antibody
Anti-Hiv-1 Vpr-Binding Ankyrin Repeat Protein antibody
Anti-Multiple Ankyrin Repeats Single Kh Domain antibody
Anti-Hmask antibody
Anti-ANKHD1 antibody
Anti-KIAA1085 antibody
Anti-MASK antibody
Anti-VBARP antibody
Anti-PP2500 antibody

Information sourced from Uniprot.org

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